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Methotrexate as an anticancer drug is hepatotoxic at high doses. It has been proven that oxidative stress is involved in methotrexate induced toxicity. Because of antioxidant potential of <em>Brassica rapa</em>. L root, this study was undertaken to examine the protective effect of hydroalcoholic extract of <em>Brasscia rapa</em> L. (BR) root  on methotrexate induced hepatotoxicity in the rat. For this purpose, forty male Wistar rats were randomly divided into four equal groups. Group 1 was used as control; groups 2 and 4 were orally treated with BR root extract (200 mg/kg) for 15 consecutive days. Groups 3 and 4 received a single intraperitoneal dose of methotrexate (20 mg/kg) on the 10th day of the experiment. At the end of the experiment, serumic levels of AST and ALT, ALP and total bilirubin, albumin and total proteins were assessed. Malondialdehyde and activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase were assayed in liver homogenates. Tissue sections were prepared from the liver and finally, the biochemical findings were compared with histopathological results. In group 4, BR root extract significantly (<em>p</em><0.05) decreased the levels of serum biomarkers of hepatic injury and total bilirubin, and significantly increased the levels of serum albumin and total proteins (<em>p</em><0.05). Also BR root extract significantly (<em>p</em><0.05) decreased the lipid peroxidation and elevated the decreased values of hepatic antioxidants in this group. Histopathologic changes including degeneration, inflammation and necrosis were in agreement with biochemical findings. The results indicated that BR root extract, because of its antioxidant potential, exerts a protective effect against methotrexate induced hepatotoxicity in rats.