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Two intergeneric hybrids of sugarcane, Co 86032 (2n=112) x Sorghum (2n=20) and Sorghum (2n=20) x S. officinarum (2n= 112), were subjected to classical as well as molecular cytological analysis. The hybridity of these clones has been confirmed by using SSR markers and chloroplast gene. During genomic in situ hybridization (GISH) the genome composition of the hybrids studied and it has revealed that it follows n+n chromosome segregation. Introgression of 10 Sorghum chromosomes in both the hybrids was observed while using Sorghum genomic DNA as the labeled probe. We report for the first time the chromosome composition and condensation behavior of Sorghum chromosomes in hybrids involving Saccharum and Sorghum.