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<p><em>Stelechocarpus burahol </em>is one of the medicinal plants that contains flavonoids. The study was carried out to know flavonoid production of cultures in vitro <em>S. burahol </em>from mesocarp explants. Mesocarp explants were cultured on MS medium containing different combination and concentration of plant growth regulators i.e. picloram (5, 7.5 and 10 mg/L) and 2, 4-D (10, 15 and 20 mg/L) under dark condition. Induction of callus formation started on the 20.29^th to the 29.86^th days. Medium supplemented with Picloram and dark state proved to be the best condition for optimum callus induction from mesocarp explants of <em>S. burahol</em>. Callus grown on medium with the addition of 7.5 mg/l Picloram produces the highest flavonoid. The maximum production of the secondary metabolite was obtained from 8 weeks old callus. However, by the time of callus ageing, its output has declined. It could be concluded that callus cultures from mesocarp <em>S. burahol</em> can be used for flavonoid production. </p><p><strong>How to Cite</strong></p><p>Habibah, N. A., Moeljopawiro, S. Dewi, K. &amp; Indrianto, A. (2016). Flavonoid Production in Callus Cultures from Mesocarp of <em>Stelechocarpus burahol</em>. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(2), 214-221.</p>