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AIM:To investigate the clinical application of Keratography 5M in the diagnosis and individualized treatment of dry eye patients. <p>METHODS:Sixteen patients who were diagnosed with dry eye in the same day were included. Tear film break-up time(BUT), lacrimal river height, and the meibomian glands were measured with a latest Keratography 5M. The patients received individualized treatment according to the examination and then recheck the items mentioned above 2wk post-treatment. The items were used for statistical analysis.<p>RESULTS:The scores of all subjects were 0.276±0.073mm for lacrimal river height, 6.343±3.304s for BUT, 3.78±1.36scores for deficiency area of meibomian glands in the first check-up and 0.292±0.065mm,8.876±3.670s,2.25±1.16scores respectively at 2wk post-treatment. The BUT and deficiency area of meibomian glands were both statistically significantly.<p>CONCLUSION:The noninvasive Keratography 5M is a conveinent and atraumatic tool in the diagnosis and treatment for dry eye. It can evaluate the treatment effectiveness objectively and systematically.