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Biogenic Synthesis of Fluorescent Carbon Dots (CDs) and Their Application in Bioimaging of Agricultural Crops
oleh: Akshay M. Pete, Pramod U. Ingle, Rajesh W. Raut, Sudhir S. Shende, Mahendra Rai, Tatiana M. Minkina, Vishnu D. Rajput, Valery P. Kalinitchenko, Aniket K. Gade
Format: | Article |
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Diterbitkan: | MDPI AG 2023-01-01 |
Deskripsi
Fluorescent nanoparticles have a transformative potential for advanced sensors and devices for point-of-need diagnostics and bioimaging, bypassing the technical burden of meeting the assay performance requirements. Carbon dots (CDs) are rapidly emerging carbon-based nanomaterials. Regardless of their fate, they will find increasing applications. In this study, a simple approach for synthesizing CDs from fruit peels was developed. The CDs were fabricated from <i>Annona squamosa</i> (L.) peels using a carbonization technique through microwave-assisted hydrothermal digestion at temperatures around 200 °C. Synthesized CDs were detected using a UV transilluminator for the preliminary confirmation of the presence of fluorescence. UV–Vis spectrophotometry (absorbance at 505 nm) analysis, zeta potential measurement (−20.8 mV), nanoparticles tracking analysis (NTA) (average size: 15.4 nm and mode size: 9.26 nm), photoluminescence, and Fourier transform infrared (FT-IR) analysis were used to identify the capping functional groups on the CDs. The total quantum yield exhibited was 8.93%, and the field emission scanning electron microscopy (FESEM) showed the size range up to 40 nm. The germinating mung bean (<i>Vigna radiata</i> (L.)) seeds were incubated with biogenically synthesized CDs to check the absorption of CDs by them. The fluorescence was observed under a UV-transilluminator in the growing parts of seeds, indicating the absorption of CDs during the germination, development, and growth. These fluorescent CDs could be used as a bioimaging agent. This novel method of synthesizing CDs was found to be eco-friendly, rapid, and cost-effective.