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Four species of reptiles, of which one is represented by one of its subspecies, are recorded for the first time from Laos: Cyrtodactylus phongnhakebangensis, Lycodon futsingensis, and L. ruhstrati, as L. ruhstrati abditus, from limestone forests in Khammouane Province and Cyrtodactylus pseudoquadrivirgatus from hill evergreen forest in Salavan Province. These discoveries of lizards and snakes bring the total species number of reptiles to 189 in Laos.