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The complex strain hardening behaviors of dual phase (DP) steel are subjected to the heterogeneous microstructures. The current work aims to predict the strain hardening behaviors of ferrite-martensite DP steel, focusing on the effects of heterogeneous microstructure on mechanical properties. The flow stress of material was calculated based on the dislocation-based work-hardening model with considering the multi-boundaries hardening. The ferrite-martensite phase boundary percent and grain shape factor were selected as the heterogeneous feature parameters, which were introduced into the new proposed model. The theoretical calculated stress-strain responses were verified with experimental results using the tensile test. The model with the boundary strengthening consideration has more accurate results. The parameter effects of ferrite-ferrite boundary (FFB) spacing, ferrite-martensite boundary (FMB) percent, and grain shape factor on the flow stress-strain curve were researched.