Disruption of Structural Disulfides of Coagulation FXIII-B Subunit; Functional Implications for a Rare Bleeding Disorder

oleh: Sneha Singh, Mohammad Suhail Akhter, Johannes Dodt, Amit Sharma, Senthilvelrajan Kaniyappan, Hamideh Yadegari, Vytautas Ivaskevicius, Johannes Oldenburg, Arijit Biswas

Format: Article
Diterbitkan: MDPI AG 2019-04-01

Deskripsi

Congenital FXIII deficiency is a rare bleeding disorder in which mutations are detected in <i>F13A1</i> and <i>F13B</i> genes that express the two subunits of coagulation FXIII, the catalytic FXIII-A, and protective FXIII-B. Mutations in FXIII-B subunit are considerably rarer compared to FXIII-A. Three mutations in the <i>F13B</i> gene have been reported on its structural disulfide bonds. In the present study, we investigate the structural and functional importance of all 20 structural disulfide bonds in FXIII-B subunit. All disulfide bonds were ablated by individually mutating one of its contributory cysteine&#8217;s, and these variants were transiently expressed in <i>HEK293t</i> cell lines. The expression products were studied for stability, secretion, the effect on oligomeric state, and on FXIII-A activation. The structural flexibility of these disulfide bonds was studied using classical MD simulation performed on a FXIII-B subunit monomer model. All 20 FXIII-B were found to be important for the secretion and stability of the protein since ablation of any of these led to a secretion deficit. However, the degree of effect that the disruption of disulfide bond had on the protein differed between individual disulfide bonds reflecting a functional hierarchy/diversity within these disulfide bonds.