A versatile, high-efficiency platform for CRISPR-based gene activation
oleh: Amy J. Heidersbach, Kristel M. Dorighi, Javier A. Gomez, Ashley M. Jacobi, Benjamin Haley
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2023-02-01 |
Deskripsi
The generation of CRISPR-mediated transcriptional activation (CRISPRa)-competent cell lines pose significant technical challenges. Here the authors report a platform for production of CRISPRa-ready cell populations which they combine with optimised expressed and synthetic gRNA scaffolds to enhance functionality.