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<i>Background and Objectives:</i> In Saudi Arabia, Acne vulgaris is a very predominant ailment among adolescents, especially female university students, and self-medication has become a trend to manage this condition. To determine the prevalence of Acne vulgaris among health care students and to access the scenario of its self-medication in light of students’ knowledge, attitude, and practice towards it. <i>Materials and Methods:</i> This was an observational study conducted at King Saud University, Riyadh, Kingdom of Saudi Arabia, from January 2022 to March 2022. The study was undertaken using a pre-structured questionnaire. <i>Results:</i> A total of 550 university students were recruited and the incidence of acne was observed to be 78.5% (432 out of 550) with a female predominance. A total of 56.0% (244 of 432) students used self-medications for acne without a prescription and the most used prescription drugs were topical and oral antibiotics (38.1%), followed by Isotretinoin (22.55), and topical adaplene (20.9%). Female students (<i>n</i> = 181, 63.5%) were significantly more likely to self-medicate compared to male students (<i>n</i> = 63, 42.9%, <i>p</i> ≤ 0.001). Almost 60% of medical students had proper knowledge of medication for acne. <i>Conclusion:</i> Acne vulgaris is a highly prevalent condition among university students of Saudi Arabia and use of self-medication among acne sufferers is high. Education programs should be made to raise awareness about acne and its treatment.