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<i>Cyclospora cayetanensis</i> is an emerging foodborne protozoan pathogen. Similar to other gastrointestinal illnesses, cyclosporiasis causes prolonged diarrhea. Unlike <i>Cryptosporidium</i>, <i>Cyclospora</i> oocysts are not infective when they are shed by infected individuals. Oocysts mature in the environment for 7&#8722;10 days before sporulating. Little is known about how <i>C. cayetanensis</i> is transported in the environment and which factors inhibit or promote sporulation. Water and fresh produce, such as leafy greens and berries, are common sources of infection. Contact with soil has also been correlated with <i>Cyclospora</i> infection. In addition to acting as a vector to transport oocysts from the environment to the body, water and soil may be important reservoirs to not only allow <i>C. cayetanensis</i> to persist, but also transport the oocysts from one location to another. This study examined a snapshot of an urban area near Chicago where human waste sporadically enters the environment via combined sewer outfalls (CSO). A total of 61 samples were collected from three CSO discharge events. Most of the 21 positive samples were wildlife feces (n = 13), and a few were soil (n = 7). There was one positive water sample. PCR analysis of soil, water, and wildlife feces indicated the presence of <i>C. cayetanensis</i> in the environment, suggesting likely transport of oocysts by wildlife. Given the emerging threat of cyclosporiasis, additional studies are needed to confirm and expand this case study.