Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information
oleh: Dacheng Ma, Zhimeng Xu, Zhaoyu Zhang, Xi Chen, Xiangzhi Zeng, Yiyang Zhang, Tingyue Deng, Mengfei Ren, Zheng Sun, Rui Jiang, Zhen Xie
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-02-01 |
Deskripsi
The genomic locations that can be targeted for editing by CRISPR are limited by the presence of the nuclease-specific PAM sequence. Here, the authors show PAM recognition can be expanded by replacing the key region in the PAM interaction domain of SaCas9 with the corresponding region of SaCas9 orthologs.