Stem cell-derived polarized hepatocytes
oleh: Viet Loan Dao Thi, Xianfang Wu, Rachel L. Belote, Ursula Andreo, Constantin N. Takacs, Joseph P. Fernandez, Luis Andre Vale-Silva, Sarah Prallet, Charlotte C. Decker, Rebecca M. Fu, Bingqian Qu, Kunihiro Uryu, Henrik Molina, Mohsan Saeed, Eike Steinmann, Stephan Urban, Roshni R. Singaraja, William M. Schneider, Sanford M. Simon, Charles M. Rice
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2020-04-01 |
Deskripsi
To model hepatocyte function accurately in vitro, it is necessary to generate and maintain a polarized epithelium. Here, the authors describe a protocol to generate polarized human pluripotent stem cell-derived hepatocyte-like cells (HLCs) to model enteric virus production and drug secretion in vitro.