An <sup>1</sup>H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail <i>Helix aspersa</i> Mucus
oleh: Nikolay G. Vassilev, Svetlana D. Simova, Miroslav Dangalov, Lyudmila Velkova, Venceslav Atanasov, Aleksandar Dolashki, Pavlinka Dolashka
| Format: | Article |
|---|---|
| Diterbitkan: | MDPI AG 2020-09-01 |
Deskripsi
Metabolic profiling based on <sup>1</sup>H nuclear magnetic resonance (NMR) spectroscopy was applied with the aim to investigate the functional role of the metabolites in lyophilized mucus from the garden snail <i>Helix aspersa</i>. Twenty metabolites were unambiguously identified by <sup>1</sup>H, 1D TOCSY, 2D J-resolved, 2D COSY, and 2D HSQC NMR spectra with water suppression. The metabolic profiles of two fractions with low molecular weight (Mw < 1 kDa and Mw < 3 kDa) are very similar. Metabolites with known antioxidant, antibacterial, and antimicrobial activity were detected by NMR metabolic analysis of mucus samples from <i>Helix aspersa</i>. Some of them were confirmed by mass spectrometric analysis. The primary structure of several peptides was identified in low molecular weight fractions (Mw < 1 kDa) by tandem mass spectrometry.