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The family Braconidae consists mostly of specialized parasitoids, some of which hold potential in biocontrol of agricultural pests. <i>Psyttalia concolor, Psyttalia humilis</i> and <i>Psyttalia lounsburyi</i> are parasitoids associated with <i>Bactrocera oleae</i>, a major pest of cultivated olives. The native range of <i>Psyttalia concolor</i> is the Mediterranean, and <i>P. humilis</i> and <i>P. lounsburyi</i> are native to sub-Saharan Africa. This study reports the mitochondrial genomes of the three species, thus laying the foundation for mitogenomic analyses in the genus <i>Psyttalia</i>. Comparative mitogenomics within Braconidae showed a novel gene arrangement in <i>Psyttalia</i> in involving translocation and inversion of transfer RNA genes. The placement of <i>Psyttalia</i> in the subfamily Opiinae was well-supported, and the divergence between <i>Psyttalia</i> and its closest relative (<i>Diachasmimorpha longicaudata</i>) was at ~55 MYA [95% highest posterior density (HPD): 34–83 MYA]. <i>Psyttalia lounsburyi</i> occupied the most basal position among the three <i>Psyttalia</i>, having diverged from the other two species ~11 MYA (95% HPD: 6–17 MYA). <i>Psyttalia concolor</i> and <i>P. humilis</i> were recovered as sister species diverged at ~2 MYA (95% HPD: 1.1–3.6 MYA). This phylogeny combining new sequences and a set of 31 other cyclostomes and non-cyclostomes highlights the importance of a comprehensive taxonomic coverage of Braconidae mitogenomes to overcome the lack of robustness in the placement of several subfamilies.