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In this paper, the notions of fuzzy subsemigroups and fuzzy bi-ideals of a po-Γ-semigroup are introduced with some of their important properties investigated. We obtain some characterizations of regular, intra-regular po-Γ-semigroups in terms of fuzzy bi-ideals. We also give a pointwise characterization of fuzzy regular subsemigroups in a po-Γ-semigroup.