A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
oleh: Wenhua Zhou, Li Hu, Liming Ying, Zhen Zhao, Paul K. Chu, Xue-Feng Yu
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2018-11-01 |
Deskripsi
Isothermal DNA amplification techniques are useful for diagnostic applications in place of traditional PCR. Here the authors describe CRISDA, which combines CRISPR–Cas9 with strand displacement amplification and exhibits attomolar sensitivity and single-nucleotide specificity in DNA detection.