FRET biosensor uncovers cAMP nano-domains at β-adrenergic targets that dictate precise tuning of cardiac contractility
oleh: Nicoletta C. Surdo, Marco Berrera, Andreas Koschinski, Marcella Brescia, Matias R. Machado, Carolyn Carr, Peter Wright, Julia Gorelik, Stefano Morotti, Eleonora Grandi, Donald M. Bers, Sergio Pantano, Manuela Zaccolo
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2017-04-01 |
Deskripsi
cAMP/PKA signalling plays important roles in physiology, but there are a lack of tools to spatially distinguish cAMP. Here the authors present a FRET-based cAMP biosensor they call CUTie that can directly compare cAMP signals at multiple subcellular sites and detect nanoscale heterogeneity in cAMP in cardiac myocytes.