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Caluromysiops irrupta Sanborn, 1951 is a poorly known didelphid marsupial species, currently known from nine localities in south-central and western Amazonia. Only two records of C. irrupta were known from Brazil. Here we report the third record, in Rondônia state, northwestern Brazil. The specimen was sighted in a well-preserved forest area, in the Parque Estadual Guajará Mirim, Guajará Mirim, Rondônia. Although this record does not represent a distribution extension, it represents an additional record for a very poorly known species.