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A new environmentally friendly flocculant was synthesized by cationic modified poly(γ-glutamic acid) (γ-PGA). The flocculant was prepared via chemical reaction between γ-PGA and 3-chloro-2-hydroxypropyl trimethyl ammonium chloride (CHPTMA). γ-PGA, as a new type of microbial flocculant with water-soluble, biodegradable, non-toxic and edible characteristics in water treatment has broad application prospects. The cationic degree of cationic modified γ-PGA was characterized by conductivity meter. Infrared spectrometry (FTIR) and 1H NMR were explored to determine chemical structure of the composite flocculant. The influence of etherification agent, reaction temperature, reaction time, as well as the molar ratio of etherifying agent and NaOH on flocculation performance were investigated. The results showed that the turbidity of the seawater distinctly decreased from 95 NTU to 10 NTU with 2 mg/L modified γ- PGA.