Inactivated herpes simplex virus-1 vaccine formulated in aqueous and alcoholic extracts of propolis boosts cellular and IgG responses

oleh: Sanaz Mojarab, Pegah Karimi, Delavar Shahbazzadeh, Majid Moghbeli, Kamran Pooshang Bagheri, Arezoo Beig Parikhani, Rada Dehghan, Ehsan Zafari, Amir Moravej, Mohammad Hassan Pouriyayevali, Seyedeh Faranak Mirtalebi, Mahdi Pakjoo, Shaghayegh Yazdani, Meghdad Abdollahpour-alitappeh, Mehdi Mahdavi

Format: Article
Diterbitkan: Mashhad University of Medical Sciences 2024-08-01

Deskripsi

Objective(s): In this study, the adjuvant activity of aqueous and alcoholic extracts of propolis was examined on the inactivated herpes simplex virus-1 (HSV-1).Materials and Methods: BALB/C mice were administered with inactivated (HSV-1; the KOS strain) plus alcoholic and aqueous extracts, followed by assessment of the cellular and humoral immune responses. Results: Alcoholic and aqueous extracts, as an adjuvant, revealed a significant increase in lymphocyte proliferation and cytotoxic T lymphocyte (CTL) responses versus the HSV-1 group. In addition, HSV-1 plus alcoholic extract showed a remarkable increase in IFN-γ cytokine and IFN-γ/IL-4 ratio. On the other hand, both alcoholic and aqueous extracts in the HSV-1 vaccine suppressed the IL-4 cytokine response as compared with the HSV-1 vaccine. In addition, HSV-1 plus alcoholic extract showed a significant increment in IgG1, IgG2a, and IgG2b isotypes as compared with the HSV-1 vaccine.Conclusion: Propolis extracts seem to modulate the immune response against inactivated HSV-1 model and can be used as a suitable vaccine adjuvant or a component of a complex adjuvant against infectious diseases.