Neurogenetic dissection of the Drosophila lateral horn reveals major outputs, diverse behavioural functions, and interactions with the mushroom body
oleh: Michael-John Dolan, Shahar Frechter, Alexander Shakeel Bates, Chuntao Dan, Paavo Huoviala, Ruairí JV Roberts, Philipp Schlegel, Serene Dhawan, Remy Tabano, Heather Dionne, Christina Christoforou, Kari Close, Ben Sutcliffe, Bianca Giuliani, Feng Li, Marta Costa, Gudrun Ihrke, Geoffrey Wilson Meissner, Davi D Bock, Yoshinori Aso, Gerald M Rubin, Gregory SXE Jefferis
| Format: | Article |
|---|---|
| Diterbitkan: | eLife Sciences Publications Ltd 2019-05-01 |
Deskripsi
Animals exhibit innate behaviours to a variety of sensory stimuli including olfactory cues. In Drosophila, one higher olfactory centre, the lateral horn (LH), is implicated in innate behaviour. However, our structural and functional understanding of the LH is scant, in large part due to a lack of sparse neurogenetic tools for this region. We generate a collection of split-GAL4 driver lines providing genetic access to 82 LH cell types. We use these to create an anatomical and neurotransmitter map of the LH and link this to EM connectomics data. We find ~30% of LH projections converge with outputs from the mushroom body, site of olfactory learning and memory. Using optogenetic activation, we identify LH cell types that drive changes in valence behavior or specific locomotor programs. In summary, we have generated a resource for manipulating and mapping LH neurons, providing new insights into the circuit basis of innate and learned olfactory behavior.