IMAPlate Based Miniature, High Sensitive, Rapid Screening Method for Detecting Bioengineered, Secreted Lipase Activities in Yeast Expression Systems
oleh: Michel A. Sciotti, Guo Jun Chen, Daniel Gygax
| Format: | Article |
|---|---|
| Diterbitkan: | Swiss Chemical Society 2010-11-01 |
Deskripsi
A spectrophotometric assay based on a miniaturized 96-well plate device (IMAPlate) enables a rapid and simple screening of bioengineered recombinant lipases expressed and secreted by Saccharomyces cerevisiae. Starting from a colony, the test delivers a quantitative estimation of enzymatic activity titer in 24 h or less with manual high throughput performances.