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In this study, GH3536 superalloy was subjected to the cold rolling processing up to 60% of thickness reductions for meticulously investigating the evolutions of both microstructure and texture. Crucial findings of deformation microstructures such as the twinning of the grain with Copper orientation and the occurrence of shear bands in matrix were captured by electron backscatter diffraction (EBSD). Twin trace analysis (TTA) was concomitant with resolved shear stress (RSS) analysis for identifying active twin variants. Higher strength and lower ductility were developed with increasing reduction ratios, which was evaluated in mechanical properties tests implemented by using nonstandard miniaturized specimens. The RSS ratio analysis was carried out to predict the twinnability of the most compliant orientation and disclose the orientation dependence of deformation twinning. Experimental and calculated results proved the discovery of the deformation twinning in a Copper orientation.