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Objective: To predict biological characterisics of 4-HppD, encoded by 4Hppd in Sporothrix schenckii. Methods: Mutiple bioinformatics online tools were used to analyse the sequence. Results: Ss.4HppD, located in mitochodria, was formed by 477 amino acids and its theoretical molecular weight is 52.0 kD. The isoelectric point was about 5.98, with a long half life. The dominant secondary structures in Ss.4HppD were random coil and alpha helix. Besides, Ss.4HppD had a similar tertiary structure with its homologue proteins, a glyoxalase motif at the carboxy end and multiple phosphorylation sites. Conclusion: Ss.4HppD was predicted to located in mitochondria, with a glyoxalase motif and various modification sites, which would facilitate the further researches in the protein including protein expression, function mechanism and inhibitior development.