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<b>Purpose:</b> To analyze the clinical and microbiological characteristics of eyes with post-traumatic endophthalmitis and factors influencing the visual outcomes in these cases. <b> Methods:</b> We performed a retrospective chart analysis of the clinical and microbiological data of 97 consecutive patients with post-traumatic endophthalmitis presenting to a tertiary eye care hospital in South India. <b> Results: </b> Thirty-nine (40.2&#x0025;) cases were culture-positive, Gram-positive cocci (n=24/42, 57.1&#x0025;) being the commonest isolates. <i> Staphylococcus epidermidis</i> and <i> Pseudomonas aeuruginosa</i> were the commonest single isolates (n=10; 23.8&#x0025;). Majority of the organisms were sensitive to chloramphenicol (n=27) or ciprofloxacin (n=26). Susceptibility to vancomycin (n=3) and amikacin (n=4) was poor. Cases with negative cultures at presentation were more likely to have improvement in visual acuity compared with culture-positive cases (on multivariate analysis, OR: 3.2, 95&#x0025; CI: 1.1, 9.0). <b> Conclusions: </b> In this series of post-traumatic endophthalmitis, a high prevalence of resistance of the culture isolates to vancomycin and amikacin was observed.