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Bioimpedance, or the electrical impedance of biological tissues, describes the passive electrical properties of these materials. To simplify bioimpedance datasets, fractional-order equivalent circuit presentations are often used, with the Cole-impedance model being one of the most widely used fractional-order circuits for this purpose. In this work, bioimpedance measurements from 10 kHz to 100 kHz were collected from participants biceps tissues immediately prior and immediately post completion of a fatiguing exercise protocol. The Cole-impedance parameters that best fit these datasets were determined using numerical optimization procedures, with relative errors of within approximately <inline-formula> <math display="inline"> <semantics> <mrow> <mo>&#177;</mo> <mn>0.5</mn> <mo>%</mo> </mrow> </semantics> </math> </inline-formula> and <inline-formula> <math display="inline"> <semantics> <mrow> <mo>&#177;</mo> <mn>2</mn> <mo>%</mo> </mrow> </semantics> </math> </inline-formula> for the simulated resistance and reactance compared to the experimental data. Comparison between the pre and post fatigue Cole-impedance parameters shows that the <inline-formula> <math display="inline"> <semantics> <msub> <mi>R</mi> <mo>&#8734;</mo> </msub> </semantics> </math> </inline-formula>, <inline-formula> <math display="inline"> <semantics> <msub> <mi>R</mi> <mn>1</mn> </msub> </semantics> </math> </inline-formula>, and <inline-formula> <math display="inline"> <semantics> <msub> <mi>f</mi> <mi>p</mi> </msub> </semantics> </math> </inline-formula> components exhibited statistically significant mean differences as a result of the fatigue induced changes in the study participants.