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Compared with the isotopes generated by nuclear reactor, the isotopes prepared by accelerator always show high specific activity and short half-life period. And most of such isotopes always were neutron-deficient nuclides, giving β+ or single energy γ ray. As one of the most important methods preparing radionuclides, the preparation of isotopes by accelerator has attracted more and more attention from the beginning of 21th century. The development of the preparation and application of the accelerator isotopes were briefly introduced in China. In addition, the forecast of the accelerator-isotope prospect were drawn.