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This study was aimed to investigate the amount of reactive blue 29 (RB29) dye removal from aqueous solution by Chitosan extracted from Persian Gulf shrimp shell. The effect of parameters such as pH, the concentration of reactive blue 29 dye, contact time, and adsorbent dosage in dye removal was studied. Isotherm, Thermodynamics, and kinetics of adsorption process were also investigated. The results of this study showed that the optimum condition of the adsorption process occurred in 50mg/L of RB29 dye concentration, pH=4, contact time of 90 minutes and adsorbent dosage of 0.2 g/L. The maximum adsorption capacity in optimum condition was estimated to 87.74 mg/g. Lab data displayed that the results were in compliance with Langmuir isotherm. According to the results of thermodynamic studies, standard entropy changes (ΔS°) was 66.67 J/mol K and standard enthalpy changes (ΔH°) was 19158.3 kJ/mol. Also, the standard Gibbs free energy (ΔG°) was negative and kinetics of adsorption process follows the pseudo-second-order model