Protein structure determination in human cells by in-cell NMR and a reporter system to optimize protein delivery or transexpression
oleh: Juan A. Gerez, Natalia C. Prymaczok, Harindranath Kadavath, Dhiman Ghosh, Matthias Bütikofer, Yanick Fleischmann, Peter Güntert, Roland Riek
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2022-12-01 |
Deskripsi
An improved method for in-cell NMR is developed using a reporter to evaluate protein delivery and delivery parameters to different cell lines and applied to IDPs and folded proteins including structure determination of GB1 at 1.1 Å resolution.