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A three days old cross bred cow calf affected with recto-vaginal fistula along with atresia ani and fusion of vulvular lips was presented to the Veterinary Teaching Hospital, College of Veterinary and Animal Sciences, Jhang. Emergency surgery was done after the infiltration of local anesthesia at the approximated site of anus. Blind rectal pouch was incised to suture it with the skin by using black breaded silk #1. A 3 inches long incision was made at the site of vulvular fusion to restore the normal genitalia. After exposing the defective site, 4 inches long slit causing direct communication between rectum and vagina (Recto-vaginal fistula) was obliterated with chromic cat gut # 1. Having washed with normal saline, Neomycine Sulphate ointment was smeared twice a day at the newly constructed anal opening for two weeks. A course of antibiotic consisting of penicillin and streptomycin was administered intramuscularly for five days. The sutures were removed on 14th post-operative day. Animal was recovered uneventfully without recurrence of any defect up to follow up of 2 months.