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We investigated the effect of <i>Trichoderma harzianum</i> in combination with biochar or other organic feedstocks, i.e., fish meal, Medicago, and maize straw, on the growth of <i>Lens culinaris</i>, <i>Zea mays</i>, <i>Oryza sativa</i>, and <i>Glycine max</i>. Biochar and other organic feedstocks were characterized by ¹³C-CPMAS NMR spectroscopy. Fish and Medicago had low C/N and high N content, while biochar, maize, and AC (Activated Carbon) had high C/N. pH ranged from 9.38 for biochar to 5.67 for AC. ¹³C-CPMAS NMR showed large chemical changes in organic mixtures leading to aromatic C-type enrichment in the presence of biochar or AC. Biochar and organic feedstocks inoculated with <i>T. harzianum</i> showed different effects, ranging from inhibition to crop stimulation. Overall, out of 88 cases, <i>T. harzianum</i> inoculum had a positive effect on root length in 46 cases (52.2%). The effect of fungal inoculum was particularly positive when combined with AC or biochar and when non-pyrogenic amendments were present. In contrast, a negative effect was observed when <i>T. harzianum</i> was inoculated with N-rich non-stabilized organic amendments. Further research is needed to identify the specific mechanisms underlying the inhibitory and bio-stimulatory effects of <i>Trichoderma</i> mixtures with organic amendment for the right combinations of raw materials that maximize crop productivity.