An achievement has been made in establishing an induced pluripotent stem cell line (SDPHi005-A) from a healthy Chinese male donor
oleh: Jing Shao, Yan Jin, Chunhong Shao, Xiaoqiao Zhang, Guang Yang
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2024-06-01 |
Deskripsi
Successfully transforming isolated peripheral blood mononuclear cells (PBMCs) from a healthy male into induced pluripotent stem cells (iPSCs) was achieved using non-integrating episomal vectors containing OCT4, SOX2, c-MYC, KLF4, and BCL-XL. The iPSCs exhibited a normal karyotype, expressed pluripotency markers, and demonstrated the ability to differentiate into three germ layers in vitro. This iPSC line may be employed for subsequent research purposes.