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Free mucosal grafts or split-thickness skin grafts have been used in patients undergoing repair procedures for oral mucosal defects. Conventional methods require the creation of second surgical wounds for use as donor sites. We applied two bilayers of artificial dermis to repair a buccal mucosal defect in one case and vestibular extension in another case. After removal of the sutures, no infection, pain, or hemorrhage developed in these patients. The results of granulation and epithelialization were good. Satisfactory appearance and function were achieved in both cases. Therefore, bilayer artificial dermis may be recommended for the repair of oral mucosal defects.