Genetic engineering for increasing resistance of chickpea (Cicer arietinum L.) to pod borer (Helicoverpa armigera)
oleh: Nasrin Moshtaghi, Abdol Reza Bagheri, T.J Higgins, Mokhtar Jalali Javaran, Behzad Ghareyazie
| Format: | Article |
|---|---|
| Diterbitkan: | Ferdowsi University of Mashhad 2010-03-01 |
Deskripsi
Pod borer is one of the main factors for yield decrease of chickpea. Therefore, breeding ofchickpea for resistance to this pest is important. Modified cry1Ac gene of Bacillus thuringiensis inpCry1Ac-nptII plasmid containing twin T-DNA for cry1Ac and nptII genes have been used fortransformation of chickpea. At last 38 plants had nptII gene that 36 plants of them had cry1Ac geneand two plants had only nptII gene. Western blotting analysis showed that 30 plants had Cry1Acprotein expression. Transformation percentage was 0.37 in this experiment. Only 7 plants produced T1seeds. Regarding to western blotting and PCR analysis for T0 and T1, plants were devided to somegroups with different expressions. Bioassay with neonate of pod borer showed high mortality in lineswith high Cry1Ac protein expression.Key words: Bt, Chickpea, cry1Ac, pod borer, transformation