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Functional Characterization of <i>PoEP1</i> in Regulating the Flowering Stage of Tree Peony
oleh: Yang Lei, Jingshan Gao, Yuying Li, Chengwei Song, Qi Guo, Lili Guo, Xiaogai Hou
Format: | Article |
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Diterbitkan: | MDPI AG 2024-06-01 |
Deskripsi
The tree peony, a traditional flower in China, has a short and concentrated flowering period, restricting the development of the tree peony industry. To explore the molecular mechanism of tree peony flowering-stage regulation, <i>PoEP1</i>, which regulated the flowering period, was identified and cloned based on the transcriptome and degradome data of the early-flowering mutant <i>Paeonia ostii</i> ‘Fengdan’ (MU) and <i>Paeonia ostii</i> ‘Fengdan’ (FD). Through bioinformatics analysis, expression pattern analysis, and transgene function verification, the role of <i>PoEP1</i> in the regulation of tree peony flowering was explored. The open-reading frame of <i>PoEP1</i> is 1161 bp, encoding 386 amino acids, containing two conserved domains. <i>PoEP1</i> was homologous to the <i>EP1</i> of other species. Subcellular localization results showed that the protein was localized in the cell wall and that <i>PoEP1</i> expression was highest in the initial decay stage of the tree peony. The overexpression of <i>PoEP1</i> in transgenic plants advanced and shortened the flowering time, indicating that <i>PoEP1</i> overexpression promotes flowering and senescence and shorten the flowering time of plants. The results of this study provide a theoretical basis for exploring the role of <i>PoEP1</i> in the regulation of tree peony flowering.