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Introduction: Patient safety care is directly affected by the training that the health care students during their academic period. There is inadequate information about the attitude of the students on this important issue in Iran. This study aimed to determine the attitude of Iranian nursing, midwifery, anesthesiology and operating room students toward patient safety. Materials and Methods:This cross-sectional study was carried out between March and July 2017 and involved 168 university students (response rate: 92.85%) who were entered in the research by stratified sampling. A valid and reliable questionnaire was used to gather the patient safety attitude of students. Data were analyzed using the SPSS, Version 16, using descriptive and analytical statistics.  Results: The mean age of the participants was 24 ± 21.17. Most of the students (57.7%) had positive attitudes towards patient safety, while 42.3% had negative attitudes. According to the results, the mean score of the students on patient safety differed based on the course of study (F=3.07, df=3, p=.03). The association between gender and semester with the attitude of the students was not significant (p>.05). Conclusion: The findings revealed that Iranian paramedical students had inadequate attitude on patient safety. It is recommended to improve the attitude of the students using suitable training programs during academic education.