High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases
oleh: Jeffrey L. Bessen, Lena K. Afeyan, Vlado Dančík, Luke W. Koblan, David B. Thompson, Chas Leichner, Paul A. Clemons, David R. Liu
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-04-01 |
Deskripsi
The development of site-specific recombinases as genome editing tools is limited by the difficulty of altering their DNA sequence specificity. Here the authors present Rec-seq, a method for identifying specificity determinants and off-target substrates of recombinases in an unbiased manner.