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Summary: In this protocol, we describe the use of RELOG, an open-source, supply chain optimization package, for robust design and analysis of optimal reverse logistics and manufacturing networks. We detail installation steps and input data assembly, followed by problem modeling. We further detail how to run the RELOG optimization, visualize, and analyze the results. The implementation discussed here illustrates battery recycling; however, our package can analyze a wide variety of supply chains with multiple types of plants, products, and time periods.For complete details on the use and execution of this protocol, please refer to Xavier and Iloeje (2020)1 and Iloeje et al. (2022).2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.