Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
oleh: Maria J. Sarmento, Michele Oneto, Simone Pelicci, Luca Pesce, Lorenzo Scipioni, Mario Faretta, Laura Furia, Gaetano Ivan Dellino, Pier Giuseppe Pelicci, Paolo Bianchini, Alberto Diaspro, Luca Lanzanò
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2018-08-01 |
Deskripsi
A known limitation of super-resolution STED microscopy is the need of high laser power which can cause photobleaching and phototoxicity. Here the authors further optimize this method and show that modulating STED intensity during acquisition results in an enhanced resolution and reduced background.