A Lotus japonicus Nodulation System Based on Heterologous Expression of the Fucosyl Transferase NodZ and the Acetyl Transferase NolL in Rhizobium leguminosarum
oleh: Cristina Pacios Bras, Meritxell Alberich Jordá, André H. M. Wijfjes, Marga Harteveld, Nico Stuurman, Jane E. Thomas-Oates, Herman P. Spaink
| Format: | Article |
|---|---|
| Diterbitkan: | The American Phytopathological Society 2000-04-01 |
Deskripsi
Heterologous expression of NodZ and NolL proteins in Rhizobium leguminosarum bv. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NolL protein obtained from Mesorhizo-bium loti functions as an acetyl transferase. We show that the NolL-dependent acetylation is specific for the fucosyl penta-N-acetylglucosamine species. In addition, the NolL protein caused elevated production of LCOs. Efficient nodulation of Lotus japonicus by the NodZ/NolL-producing strain was demonstrated. Nodulation efficiency was further improved by the addition of the ethylene inhibitor L-α-(2-aminoethoxyvinyl) glycine (AVG).