Six New Methyl Apiofuranosides from the Bark of <i>Phellodendron chinense</i> Schneid and Their Inhibitory Effects on Nitric Oxide Production
oleh: Peng-Fei Wang, Yan-Ping Li, Li-Qin Ding, Shi-Jie Cao, Li-Ning Wang, Feng Qiu
| Format: | Article |
|---|---|
| Diterbitkan: | MDPI AG 2019-05-01 |
Deskripsi
A chemical investigation on 70% EtOH extract from the bark of <i>Phellodendron chinense</i> Schneid (Rutaceae) led to six new methyl apiofuranosides (<b>1</b>–<b>6</b>), and ten known compounds (<b>7</b>–<b>16</b>). All these compounds were characterized by the basic analysis of the spectroscopic data including extensive 1D-, 2D-NMR (HSQC, HMBC), and high-resolution mass spectrometry, and the absolute configurations were determined by both empirical approaches and NOESY. Inhibitory effects of compounds <b>1</b>–<b>9</b> and <b>11</b>–<b>16</b> on nitric oxide production were investigated in lipopolysaccharide (LPS)-mediated RAW 264.7 cells, as a result, most of these isolates inhibited nitric oxide (NO) release, and among them <b>9</b>, <b>11,</b> and <b>12</b> displayed the strongest inhibition on NO release at the concentration of 12.5 μM.