Low-dose phase retrieval of biological specimens using cryo-electron ptychography
oleh: Liqi Zhou, Jingdong Song, Judy S. Kim, Xudong Pei, Chen Huang, Mark Boyce, Luiza Mendonça, Daniel Clare, Alistair Siebert, Christopher S. Allen, Emanuela Liberti, David Stuart, Xiaoqing Pan, Peter D. Nellist, Peijun Zhang, Angus I. Kirkland, Peng Wang
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2020-06-01 |
Deskripsi
Cryo-electron microscopy is widely employed in structural biology and uses phase contrast imaging. Here, the authors employ electron ptychography, a quantitative phase retrieval method for high-contrast, low-dose phase imaging of cryo-state rotavirus and immature HIV-1 virus-like particles, and show that electron ptychography is more efficient for phase recovery than conventional phase contrast imaging.