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The reliable estimation of ionospheric refraction effects is an important topic in the GNSS (Global Navigation Satellite Systems) positioning and navigation domain, especially in safety-of-life applications. This paper describes a three-dimensional ionosphere reconstruction approach that combines three data sources with an ionospheric background model: space- and ground-based total electron content (TEC) measurements and ionosonde observations. First the background model is adjusted by F2 layer characteristics, obtained from space-based ionospheric radio occultation (IRO) profiles and ionosonde data, and secondly the final electron density distribution is estimated by an algebraic reconstruction technique.<br><br>The method described is validated by TEC measurements of independent ground-based GNSS stations, space-based TEC from the Jason 1 and 2 satellites, and ionosonde observations. A significant improvement is achieved by the data assimilation, with a decrease in the residual errors by up to 98 % compared to the initial guess of the background. Furthermore, the results underpin the capability of space-based measurements to overcome data gaps in reconstruction areas where less GNSS ground-station infrastructure exists.