Targeted removal of epigenetic barriers during transcriptional reprogramming

oleh: Valentin Baumann, Maximilian Wiesbeck, Christopher T. Breunig, Julia M. Braun, Anna Köferle, Jovica Ninkovic, Magdalena Götz, Stefan H. Stricker

Format: Article
Diterbitkan: Nature Portfolio 2019-05-01

Deskripsi

Master transcription factors dominantly direct cell fate and barriers ensuring their tissue specific silencing are not clearly defined. Here, the authors demonstrate that inefficient targeted transactivation of Sox1 in neural progenitor cells is surmountable through targeted promoter demethylation using dCas9-Tet1.