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The global demand for plastics has been continuously increasing at a rapid rate and their disposal largely pollutes the atmosphere and oceans. Natural fibers possess the inherent characteristics required for plastic making and their disposal causes less harm to the environment. In this paper, physicochemical, thermal, mechanical and morphological characteristics of novel cellulosic fiber extracted from the bark of Tithonia diversifolia (TD) investigated through various characterization tests have been reported. TDFs were found to possess higher cellulose (63.9 wt.%) and high lignin content (19.3 wt.%) which make them stronger and more stable. Lower density (1440 kg/m3), higher crystallinity index (51.84%), higher tensile strength (243–386 MPa) and higher thermal stability (up to 337.17°C) make TDFs more suitable for making green composites. The morphology of the TD fiber surface observed through field emission scanning electron microscopy (FESEM) was found to be rougher with some serrations which facilitate better bonding characteristics with the polymer matrix.