Modifications to the classical rat aortic ring model to allow vascular degeneration studies
oleh: Guillaume Blot, Thérèse-Marie Sartoris, Florian Sennlaub, Xavier Guillonneau
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2021-03-01 |
Deskripsi
Summary: The classical aortic ring model is well suited for deciphering pro-angiogenic processes. Here, we propose simple modifications of the standard protocol to study various anti-angiogenic processes from growth arrest to capillary degeneration. Aortic rings are cultured under basal conditions for 6 days to allow physiological vessel sprouting and then split into treatment groups to follow capillary growth or degeneration for an additional 2 days.