Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells
oleh: Gavuthami Murugesan, Lindsay Davidson, Linda Jannetti, Paul R. Crocker, Bernd Weigle
| Format: | Article |
|---|---|
| Diterbitkan: | MDPI AG 2022-01-01 |
Deskripsi
Macrophages (M<sub>Φ</sub>) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated M<sub>Φ</sub> can exist in two extreme phenotypes: pro-inflammatory (M1) M<sub>Φ</sub> and anti-inflammatory (M2) M<sub>Φ</sub>. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived M<sub>Φ</sub> have gained major attention, as they are functionally similar to human monocyte-derived M<sub>Φ</sub> and are receptive to genome editing. In this study, we polarised iPSC-derived M<sub>Φ</sub> to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised M<sub>Φ</sub>.