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Design of collimators and their effectiveness for the purposes of the fractionated mini-beam hadron radiation therapy were evaluated by Monte Carlo simulations. The calculations have been performed for proton, carbon and oxygen ion beams at the energies relevant for medical applications. Micropixel metal and hybrid detectors were tested for measuring charged particles intensity distribution in multi-beam structures shaped by slit or matrix collimators exploring low energy proton beam at the Tandem generator (INR NASU, Kyiv). The results obtained illustrate reliable performance of the designed collimators as well as hybrid and metal microdetectors for measuring and imaging in real time the proton intensity distribution over mini-beam structures.