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This research aimed to evaluate the performance of dyes extracted from Acacia Nilotica bark on Tencel fabric dyeing. The extraction of dye was optimized by extracting dyes with different liquor ratios. The dyeing parameters were optimized under pH values from 5 to 14. The effect of different mordants was also observed to see the shades this dye can produce on Tencel fabric. The optimum dye extraction conditions for material-to-liquor ratio were found to be 1:10. The optimum pH was found to be 12. The color yield and fastness characteristics of the dyed materials were measured to assess them. It was determined that the addition of mordants affected the color values; as a result, distinct fashion hues were produced from the same dye extract by employing various mordants. This basic study of dyeing and extracting natural dye showed that this dye has the potential to be used for cellulosic fibers.